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96-well high-affinity binding microplate costar assay plate  (Corning Life Sciences)

 
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    Corning Life Sciences 96-well high-affinity binding microplate costar assay plate
    96 Well High Affinity Binding Microplate Costar Assay Plate, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+binding+affinity+96/96+well+high+affinity+binding+microplate+costar+assay+plate/pm40172224-164-2-8
    Average 90 stars, based on 1 article reviews
    96-well high-affinity binding microplate costar assay plate - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Estrogen receptor-α signaling in tanycytes lies at the crossroads of fertility and metabolism.
    Article Snippet: Briefly, 96-well high-affinity binding plates (CLS3369, Corning®) were coated with a bovine LHβ518B7 monoclonal antibody (1:1,000 in PBS; 50μl per well).

    Article Title: Treatment of women affected with polycystic ovary syndrome
    Article Snippet: A 96-well high-affinity binding microplate (Corning) was coated with 50 μl of capture antibody (monoclonal antibody, anti-bovine LH beta subunit, 518B7; University of California, Lillian Sibley UC Davis) at a final dilution of 1:1000 (in Na2CO3/NaHCO3, 0.1M, pH 9.6) and incubated overnight at 4° C. Wells were incubated with 200 μl of blocking buffer (5% (w/v) skim milk powder in 1×PBS-T (0.01M PBS, 1.09 g of Na2HPO4 (anhydrous), 0.32 g of NaH2PO4 (anhydrous) and 9g NaCl in 1 L distilled water with 0.05% Tween 20 (pH 7.4, Sigma #P9416)) for 2 hr at RT.

    Article Title: Enhanced durability of a Zika virus self-amplifying RNA vaccine through combinatorial OX40 and 4-1BB agonism.
    Article Snippet: 474 475 Enzyme-linked immunosorbent assay 476 High-binding affinity ELISA plates (96-well; Corning, #9018) were coated with ZIKV E protein (2 477 mg/ml, Native Antigen, #ZIKVSU-ENV) in coating buffer (0.1 MNaHCO3) overnight at 4°C and 478 then blocked for 1 h at room temperature with 5% Blocker Casein in PBS (Thermo Fisher 479 Scientific, #37528).

    Article Title: The preoptic Kisspeptin/nNOS/GnRH (KiNG) neuronal network regulates rhythmic LH release through a dual activation-inhibition mechanism
    Article Snippet: Briefly, we used a 96-well high-affinity binding microplate (Corning) coated with 50 μL of primary capture antibody (bovine LHβ subunit, 518B7; L. Sibley; University of California, UC Davis) diluted at 1μg/ml in 0.01M PBS.

    Article Title: Enhanced durability of a Zika virus self-amplifying RNA vaccine through combinatorial OX40 and 4-1BB agonism
    Article Snippet: High-binding affinity ELISA plates (96-well; Corning, 9018) were coated with ZIKV E protein (2 mg/mL, Native Antigen, ZIKVSU-ENV) in coating buffer (0.1 MNaHCO 3 ) overnight at 4°C and then blocked for 1 hour at room temperature with 5% Blocker Casein in PBS (Thermo Fisher Scientific, 37528).

    Article Title: Preventing and correcting polycystic ovary syndrome by targeting anti-Müllerian hormone signaling in minipuberty and adulthood in mice.
    Article Snippet: For this study, we used an ultrasensitive ELISA method to measure circulating LH levels in the whole blood in mice as validated previously to assess pulsatile LH release in femalemice over the estrous cycle.37,38 Briefly, this ELISA uses a 96-well high-affinity binding microplate (Corning) coatedwith 50 mL of primary capture antibody (bovine LHb subunit, 518B7; L. Sibley; University of California, UC Davis) at a dilution of 1:1000.

    Article Title: Role of membrane estrogen receptor alpha on the positive feedback of estrogens on Kisspeptin and GnRH neurons
    Article Snippet: Briefly, 96-well high-affinity binding microplates (9018, Corning) were coated with 50 μl of a monoclonal antibody directed against bovine LH beta subunit (1:1,000; 518B7; RRID: AB_2665514, University of California, UC Davis) and incubated overnight at 4°C.

    Article Title: Human coronavirus OC43-elicited CD4 + T cells protect against SARS-CoV-2 in HLA transgenic mice
    Article Snippet: High-binding affinity 96-well plates (Costar) were coated overnight with 1 μg/mL of recombinant SARS-CoV-2 S or N protein or OC43 S protein (Sino Biological, 40607-V08B), and then blocked with 5% blotting-grade casein (Bio-Rad).

    Binding Assay:

    Article Title: Estrogen receptor-α signaling in tanycytes lies at the crossroads of fertility and metabolism.
    Article Snippet: Briefly, 96-well high-affinity binding plates (CLS3369, Corning®) were coated with a bovine LHβ518B7 monoclonal antibody (1:1,000 in PBS; 50μl per well).

    Article Title: Treatment of women affected with polycystic ovary syndrome
    Article Snippet: A 96-well high-affinity binding microplate (Corning) was coated with 50 μl of capture antibody (monoclonal antibody, anti-bovine LH beta subunit, 518B7; University of California, Lillian Sibley UC Davis) at a final dilution of 1:1000 (in Na2CO3/NaHCO3, 0.1M, pH 9.6) and incubated overnight at 4° C. Wells were incubated with 200 μl of blocking buffer (5% (w/v) skim milk powder in 1×PBS-T (0.01M PBS, 1.09 g of Na2HPO4 (anhydrous), 0.32 g of NaH2PO4 (anhydrous) and 9g NaCl in 1 L distilled water with 0.05% Tween 20 (pH 7.4, Sigma #P9416)) for 2 hr at RT.

    Article Title: Enhanced durability of a Zika virus self-amplifying RNA vaccine through combinatorial OX40 and 4-1BB agonism.
    Article Snippet: 474 475 Enzyme-linked immunosorbent assay 476 High-binding affinity ELISA plates (96-well; Corning, #9018) were coated with ZIKV E protein (2 477 mg/ml, Native Antigen, #ZIKVSU-ENV) in coating buffer (0.1 MNaHCO3) overnight at 4°C and 478 then blocked for 1 h at room temperature with 5% Blocker Casein in PBS (Thermo Fisher 479 Scientific, #37528).

    Article Title: The preoptic Kisspeptin/nNOS/GnRH (KiNG) neuronal network regulates rhythmic LH release through a dual activation-inhibition mechanism
    Article Snippet: Briefly, we used a 96-well high-affinity binding microplate (Corning) coated with 50 μL of primary capture antibody (bovine LHβ subunit, 518B7; L. Sibley; University of California, UC Davis) diluted at 1μg/ml in 0.01M PBS.

    Article Title: Enhanced durability of a Zika virus self-amplifying RNA vaccine through combinatorial OX40 and 4-1BB agonism
    Article Snippet: High-binding affinity ELISA plates (96-well; Corning, 9018) were coated with ZIKV E protein (2 mg/mL, Native Antigen, ZIKVSU-ENV) in coating buffer (0.1 MNaHCO 3 ) overnight at 4°C and then blocked for 1 hour at room temperature with 5% Blocker Casein in PBS (Thermo Fisher Scientific, 37528).

    Article Title: Preventing and correcting polycystic ovary syndrome by targeting anti-Müllerian hormone signaling in minipuberty and adulthood in mice.
    Article Snippet: For this study, we used an ultrasensitive ELISA method to measure circulating LH levels in the whole blood in mice as validated previously to assess pulsatile LH release in femalemice over the estrous cycle.37,38 Briefly, this ELISA uses a 96-well high-affinity binding microplate (Corning) coatedwith 50 mL of primary capture antibody (bovine LHb subunit, 518B7; L. Sibley; University of California, UC Davis) at a dilution of 1:1000.

    Article Title: Role of membrane estrogen receptor alpha on the positive feedback of estrogens on Kisspeptin and GnRH neurons
    Article Snippet: Briefly, 96-well high-affinity binding microplates (9018, Corning) were coated with 50 μl of a monoclonal antibody directed against bovine LH beta subunit (1:1,000; 518B7; RRID: AB_2665514, University of California, UC Davis) and incubated overnight at 4°C.

    Article Title: Human coronavirus OC43-elicited CD4 + T cells protect against SARS-CoV-2 in HLA transgenic mice
    Article Snippet: High-binding affinity 96-well plates (Costar) were coated overnight with 1 μg/mL of recombinant SARS-CoV-2 S or N protein or OC43 S protein (Sino Biological, 40607-V08B), and then blocked with 5% blotting-grade casein (Bio-Rad).

    Incubation:

    Article Title: Estrogen receptor-α signaling in tanycytes lies at the crossroads of fertility and metabolism.
    Article Snippet: Briefly, 96-well high-affinity binding plates (CLS3369, Corning®) were coated with a bovine LHβ518B7 monoclonal antibody (1:1,000 in PBS; 50μl per well).

    Article Title: Treatment of women affected with polycystic ovary syndrome
    Article Snippet: A 96-well high-affinity binding microplate (Corning) was coated with 50 μl of capture antibody (monoclonal antibody, anti-bovine LH beta subunit, 518B7; University of California, Lillian Sibley UC Davis) at a final dilution of 1:1000 (in Na2CO3/NaHCO3, 0.1M, pH 9.6) and incubated overnight at 4° C. Wells were incubated with 200 μl of blocking buffer (5% (w/v) skim milk powder in 1×PBS-T (0.01M PBS, 1.09 g of Na2HPO4 (anhydrous), 0.32 g of NaH2PO4 (anhydrous) and 9g NaCl in 1 L distilled water with 0.05% Tween 20 (pH 7.4, Sigma #P9416)) for 2 hr at RT.

    Article Title: Enhanced durability of a Zika virus self-amplifying RNA vaccine through combinatorial OX40 and 4-1BB agonism.
    Article Snippet: 474 475 Enzyme-linked immunosorbent assay 476 High-binding affinity ELISA plates (96-well; Corning, #9018) were coated with ZIKV E protein (2 477 mg/ml, Native Antigen, #ZIKVSU-ENV) in coating buffer (0.1 MNaHCO3) overnight at 4°C and 478 then blocked for 1 h at room temperature with 5% Blocker Casein in PBS (Thermo Fisher 479 Scientific, #37528).

    Article Title: The preoptic Kisspeptin/nNOS/GnRH (KiNG) neuronal network regulates rhythmic LH release through a dual activation-inhibition mechanism
    Article Snippet: Briefly, we used a 96-well high-affinity binding microplate (Corning) coated with 50 μL of primary capture antibody (bovine LHβ subunit, 518B7; L. Sibley; University of California, UC Davis) diluted at 1μg/ml in 0.01M PBS.

    Article Title: Enhanced durability of a Zika virus self-amplifying RNA vaccine through combinatorial OX40 and 4-1BB agonism
    Article Snippet: High-binding affinity ELISA plates (96-well; Corning, 9018) were coated with ZIKV E protein (2 mg/mL, Native Antigen, ZIKVSU-ENV) in coating buffer (0.1 MNaHCO 3 ) overnight at 4°C and then blocked for 1 hour at room temperature with 5% Blocker Casein in PBS (Thermo Fisher Scientific, 37528).

    Article Title: Preventing and correcting polycystic ovary syndrome by targeting anti-Müllerian hormone signaling in minipuberty and adulthood in mice.
    Article Snippet: For this study, we used an ultrasensitive ELISA method to measure circulating LH levels in the whole blood in mice as validated previously to assess pulsatile LH release in femalemice over the estrous cycle.37,38 Briefly, this ELISA uses a 96-well high-affinity binding microplate (Corning) coatedwith 50 mL of primary capture antibody (bovine LHb subunit, 518B7; L. Sibley; University of California, UC Davis) at a dilution of 1:1000.

    Article Title: Role of membrane estrogen receptor alpha on the positive feedback of estrogens on Kisspeptin and GnRH neurons
    Article Snippet: Briefly, 96-well high-affinity binding microplates (9018, Corning) were coated with 50 μl of a monoclonal antibody directed against bovine LH beta subunit (1:1,000; 518B7; RRID: AB_2665514, University of California, UC Davis) and incubated overnight at 4°C.

    Article Title: Human coronavirus OC43-elicited CD4 + T cells protect against SARS-CoV-2 in HLA transgenic mice
    Article Snippet: High-binding affinity 96-well plates (Costar) were coated overnight with 1 μg/mL of recombinant SARS-CoV-2 S or N protein or OC43 S protein (Sino Biological, 40607-V08B), and then blocked with 5% blotting-grade casein (Bio-Rad).

    Blocking Assay:

    Article Title: Estrogen receptor-α signaling in tanycytes lies at the crossroads of fertility and metabolism.
    Article Snippet: Briefly, 96-well high-affinity binding plates (CLS3369, Corning®) were coated with a bovine LHβ518B7 monoclonal antibody (1:1,000 in PBS; 50μl per well).

    Article Title: Treatment of women affected with polycystic ovary syndrome
    Article Snippet: A 96-well high-affinity binding microplate (Corning) was coated with 50 μl of capture antibody (monoclonal antibody, anti-bovine LH beta subunit, 518B7; University of California, Lillian Sibley UC Davis) at a final dilution of 1:1000 (in Na2CO3/NaHCO3, 0.1M, pH 9.6) and incubated overnight at 4° C. Wells were incubated with 200 μl of blocking buffer (5% (w/v) skim milk powder in 1×PBS-T (0.01M PBS, 1.09 g of Na2HPO4 (anhydrous), 0.32 g of NaH2PO4 (anhydrous) and 9g NaCl in 1 L distilled water with 0.05% Tween 20 (pH 7.4, Sigma #P9416)) for 2 hr at RT.

    Article Title: Enhanced durability of a Zika virus self-amplifying RNA vaccine through combinatorial OX40 and 4-1BB agonism.
    Article Snippet: 474 475 Enzyme-linked immunosorbent assay 476 High-binding affinity ELISA plates (96-well; Corning, #9018) were coated with ZIKV E protein (2 477 mg/ml, Native Antigen, #ZIKVSU-ENV) in coating buffer (0.1 MNaHCO3) overnight at 4°C and 478 then blocked for 1 h at room temperature with 5% Blocker Casein in PBS (Thermo Fisher 479 Scientific, #37528).

    Article Title: The preoptic Kisspeptin/nNOS/GnRH (KiNG) neuronal network regulates rhythmic LH release through a dual activation-inhibition mechanism
    Article Snippet: Briefly, we used a 96-well high-affinity binding microplate (Corning) coated with 50 μL of primary capture antibody (bovine LHβ subunit, 518B7; L. Sibley; University of California, UC Davis) diluted at 1μg/ml in 0.01M PBS.

    Article Title: Enhanced durability of a Zika virus self-amplifying RNA vaccine through combinatorial OX40 and 4-1BB agonism
    Article Snippet: High-binding affinity ELISA plates (96-well; Corning, 9018) were coated with ZIKV E protein (2 mg/mL, Native Antigen, ZIKVSU-ENV) in coating buffer (0.1 MNaHCO 3 ) overnight at 4°C and then blocked for 1 hour at room temperature with 5% Blocker Casein in PBS (Thermo Fisher Scientific, 37528).

    Article Title: Preventing and correcting polycystic ovary syndrome by targeting anti-Müllerian hormone signaling in minipuberty and adulthood in mice.
    Article Snippet: For this study, we used an ultrasensitive ELISA method to measure circulating LH levels in the whole blood in mice as validated previously to assess pulsatile LH release in femalemice over the estrous cycle.37,38 Briefly, this ELISA uses a 96-well high-affinity binding microplate (Corning) coatedwith 50 mL of primary capture antibody (bovine LHb subunit, 518B7; L. Sibley; University of California, UC Davis) at a dilution of 1:1000.

    Article Title: Role of membrane estrogen receptor alpha on the positive feedback of estrogens on Kisspeptin and GnRH neurons
    Article Snippet: Briefly, 96-well high-affinity binding microplates (9018, Corning) were coated with 50 μl of a monoclonal antibody directed against bovine LH beta subunit (1:1,000; 518B7; RRID: AB_2665514, University of California, UC Davis) and incubated overnight at 4°C.

    Article Title: Human coronavirus OC43-elicited CD4 + T cells protect against SARS-CoV-2 in HLA transgenic mice
    Article Snippet: High-binding affinity 96-well plates (Costar) were coated overnight with 1 μg/mL of recombinant SARS-CoV-2 S or N protein or OC43 S protein (Sino Biological, 40607-V08B), and then blocked with 5% blotting-grade casein (Bio-Rad).



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